The Donald Danforth Plant Science Center Bioinformatics facility is a physical and intellectual infrastructure that facilitates the bioinformatic computing and storage needs of members of the Danforth Center community. The physical core is a modular, virtualized cloud-based framework that provides the foundation for expansion of computing and storage resources by the Danforth Center and individual labs or groups. The current infrastructure contains 424 processing cores, >2 terabytes of memory, and a single, high-performance 204 terabyte file server. These resources are shared in a managed, multi-user environment and tied together by a 10 Gigabit network. Management of the system is simplified through virtualization of server resources, which also allows for the deployment of diverse applications or platforms simultaneously. The Bioinformatics facility also provides a framework for intellectual development through regular workshops and training events, custom application development for lab or group projects and community-based sharing of tools and ideas. In addition, the Bioinformatics facility enhances interaction between groups at the center and partner institutions, such as Washington University in St. Louis.